Graves S, Drummond L, Strugnell R
Sex Transm Dis. 1984 Jan-Mar;11(1):1-5. doi: 10.1097/00007435-198401000-00001.
Rabbits were immunized with viable Spirochaeta aurantia, a free-living, facultative anaerobic spirochete that is similar in some biochemical characteristics to Treponema pallidum, a parasitic, microaerophilic spirochete. Single and multiple immunizations with living S. aurantia, with or without Freund's incomplete adjuvant, Freund's complete adjuvant, or heat-killed T. pallidum, were carried out over a four-month period. Living S. aurantia was neither toxic nor virulent for rabbits. Immunized rabbits produced a high level of agglutinating antibody to S. aurantia but no antibody to T. pallidum, as determined by the T. pallidum hemagglutination test. Immunized rabbits were challenged with multiple intradermal inoculations of 100 viable T. pallidum (Nichols strain) and compared to unimmunized rabbits similarly infected. Immunization with S. aurantia did not protect against T. pallidum infection. Thus S. aurantia appears not to be suitable as a potential vaccine against infection with T. pallidum.
用活的橙色螺旋体免疫兔子，橙色螺旋体是一种自由生活的兼性厌氧螺旋体，在某些生化特性上与梅毒螺旋体相似，梅毒螺旋体是一种寄生的微需氧螺旋体。在四个月的时间里，用活的橙色螺旋体进行单次和多次免疫，使用或不使用弗氏不完全佐剂、弗氏完全佐剂或热灭活的梅毒螺旋体。活的橙色螺旋体对兔子既无毒性也无致病性。通过梅毒螺旋体血凝试验测定，免疫兔子产生了高水平的针对橙色螺旋体的凝集抗体，但未产生针对梅毒螺旋体的抗体。对免疫兔子进行多次皮内接种100个活的梅毒螺旋体（Nichols株）的攻击，并与同样感染的未免疫兔子进行比较。用橙色螺旋体免疫不能预防梅毒螺旋体感染。因此，橙色螺旋体似乎不适合作为预防梅毒螺旋体感染的潜在疫苗。
