Maréchal L R
Arch Microbiol. 1984 Jan;137(1):70-3. doi: 10.1007/BF00425810.
The metabolism of trehalose in wild type cells of Escherichia coli and Salmonella typhimurium has been investigated. Intact cells of Escherichia coli (grown on trehalose) accumulated [14C]-trehalose as [14C]-trehalose 6-phosphate. Toluene-treated cells catalyzed the synthesis of the [14C]-sugar phosphate from [14C]-trehalose and phosphoenolpyruvate; ATP did not serve as phosphoryl donor. Trehalose 6-phosphate could subsequently be hydrolyzed by trehalose 6-phosphate hydrolase, an enzyme which catalyzes the hydrolysis of the disaccharide phosphate into glucose and glucose 6-phosphate. Both Escherichia coli and Salmonella typhimurium induced this enzyme when they grew on trehalose. These findings suggest that trehalose is transported in these bacteria by an inducible phosphoenolpyruvate:trehalose phosphotransferase system. The presence of a constitutive trehalase was also detected.
对大肠杆菌和鼠伤寒沙门氏菌野生型细胞中海藻糖的代谢进行了研究。完整的大肠杆菌细胞（在海藻糖上生长）将[14C] - 海藻糖积累为[14C] - 海藻糖6 - 磷酸。经甲苯处理的细胞催化由[14C] - 海藻糖和磷酸烯醇丙酮酸合成[14C] - 糖磷酸；ATP不作为磷酰基供体。海藻糖6 - 磷酸随后可被海藻糖6 - 磷酸水解酶水解，该酶催化二糖磷酸水解为葡萄糖和葡萄糖6 - 磷酸。当大肠杆菌和鼠伤寒沙门氏菌在海藻糖上生长时，它们都会诱导产生这种酶。这些发现表明，海藻糖在这些细菌中是通过可诱导的磷酸烯醇丙酮酸：海藻糖磷酸转移酶系统进行转运的。还检测到了组成型海藻糖酶的存在。
