Shlopov V G, Krivenko N A, Shevchenko T I
Arkh Patol. 1984;46(2):80-1.
The quality of paraffin sections is determined to a considerable degree by the properties of paraffin block such as crystal size and spatial homogeneity. A new temperature regimen of treatment in preparation of paraffin blocks has been developed. After impregnation, pieces of tissue are overlayed with paraffin at a temperature of 56-60 degrees C in paper-tin foil dishes and then immediately placed in an antifreeze at 0 degree to -5 degrees C. The internal stresses, deformations, and microcracks occurring in this procedure are eliminated by incubation of the resulting block in a thermostat at 45 degrees C in vapours of low-melting fraction of paraffin (2-24 hours) followed by rapid cooling with cold air (from -5 degrees to -10 degrees C). The second step of the treatment may also be used for improvement of the quality of paraffin blocks after long-term storage.
石蜡切片的质量在很大程度上取决于石蜡块的性质，如晶体大小和空间均匀性。已开发出一种制备石蜡块的新温度处理方案。浸渍后，将组织块在56 - 60摄氏度的温度下于纸锡箔盘中用石蜡覆盖，然后立即放入0摄氏度至 - 5摄氏度的防冻液中。通过将所得蜡块在45摄氏度的恒温箱中置于低熔点石蜡馏分的蒸汽中孵育（2 - 24小时），随后用冷空气快速冷却（从 - 5摄氏度至 - 10摄氏度），消除该过程中产生的内应力、变形和微裂纹。处理的第二步也可用于改善长期储存后石蜡块的质量。
