Kozik M B, Szczech J, Głowacka A
Folia Histochem Cytochem (Krakow). 1979;17(1):77-83.
The possibility of demonstrating the activity of respiratory enzymes in paraffin sections was studied. Unfixed pieces of nervous tissue were incubated at 4 degrees C, 20 degrees C, 37 degrees C and 56 degrees C for various periods ranging from 1 to 24 hours. After dehydration, the tissue pieces were mounted in paraffin. The paraffin sections obtained there of were then tested with respect to the range of penetration of the substrate into the incubated tissue samples (as judged from the resulting histoenzymic reaction), and for the distinctness with which the localization of the histochemic reaction could be assessed. From the results it may be concluded that it is possible, under well defined conditions, to demonstrate the activity of dehydrogenases in paraffin sections. The resulting morphological pictures permit a much better localization of the histoenzymic reaction products than those obtained from cryostat sections. Optimal results are obtained when tissue fragments, about 1 mm in diameter are incubated for 24 hours at 4 degrees C.
研究了在石蜡切片中显示呼吸酶活性的可能性。将未固定的神经组织块在4℃、20℃、37℃和56℃下孵育1至24小时不等的不同时间段。脱水后，将组织块包埋在石蜡中。然后对由此获得的石蜡切片进行测试，以确定底物渗入孵育组织样本的范围（根据产生的组织酶反应判断），以及评估组织化学反应定位的清晰度。从结果可以得出结论，在明确界定的条件下，有可能在石蜡切片中显示脱氢酶的活性。与冷冻切片相比，所得的形态学图像能更好地定位组织酶反应产物。当直径约1毫米的组织碎片在4℃下孵育24小时时，可获得最佳结果。
