人巨噬细胞系U937上C1q受体的特性研究。
Characterization of the C1q receptor on a human macrophage cell line, U937.
作者信息
Arvieux J, Reboul A, Bensa J C, Colomb M G
出版信息
Biochem J. 1984 Mar 1;218(2):547-55. doi: 10.1042/bj2180547.
Abstract
The binding of C1q to the human macrophage cell line U937 has been studied. Fluorescence microscopy with fluorescein-conjugated F(ab')2 anti-C1q antibody showed that 100% of the cell population is able to bind exogenous C1q. Monomeric C1q binding to U937 cells is very weak at normal ionic strength (I0.15) and was therefore investigated at I0.07, conditions which stabilize the binding. However, aggregation of C1q on dextran sulphate or a lipid A-rich lipopolysaccharide allowed a firm, binding at I0.15. Quantitative binding studies with monomeric 125I-C1q showed a concentration-dependent, saturable, specific and reversible binding involving specific membrane receptors. Scatchard plots of C1q binding indicated [1.6 +/- 0.7 (1 S.D.)] X 10(6) sites per cell with an equilibrium constant of (2.9 +/- 1.8) X 10(7) M-1 at I0.07. The location of the molecule region mediating C1q binding was established with collagen-like fragments prepared by partial pepsin digestion, confirming earlier results obtained by inhibition studies.
摘要
已经对C1q与人巨噬细胞系U937的结合进行了研究。用荧光素偶联的F(ab')2抗C1q抗体进行荧光显微镜检查表明，100%的细胞群体能够结合外源性C1q。在正常离子强度（I0.15）下，单体C1q与U937细胞的结合非常弱，因此在I0.07（稳定结合的条件）下进行了研究。然而，C1q在硫酸葡聚糖或富含脂质A的脂多糖上的聚集允许在I0.15下牢固结合。用单体125I-C1q进行的定量结合研究表明，存在涉及特定膜受体的浓度依赖性、可饱和、特异性和可逆结合。C1q结合的Scatchard图表明，在I0.07时，每个细胞有[1.6±0.7（1个标准差）]×10(6)个位点，平衡常数为(2.9±1.8)×10(7) M-1。通过部分胃蛋白酶消化制备的胶原样片段确定了介导C1q结合的分子区域的位置，证实了早期通过抑制研究获得的结果。
相似文献
1
Characterization of the C1q receptor on a human macrophage cell line, U937.人巨噬细胞系U937上C1q受体的特性研究。
Biochem J. 1984 Mar 1;218(2):547-55. doi: 10.1042/bj2180547.
2
Characterization of the binding of purified human C1q to heart mitochondrial membranes.纯化的人C1q与心脏线粒体膜结合的特性研究。
J Biol Chem. 1981 Nov 10;256(21):10924-9.
3
C1Q receptors on cultured human gingival fibroblasts: analysis of binding properties.培养的人牙龈成纤维细胞上的C1Q受体：结合特性分析
J Immunol. 1983 Apr;130(4):1871-5.
4
Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q.一种与C1q球状“头部”结合的33-kD细胞表面糖蛋白的分离、cDNA克隆及过表达
J Exp Med. 1994 Jun 1;179(6):1809-21. doi: 10.1084/jem.179.6.1809.
5
Both IgG- and C1q-receptors play a role in the enhanced binding of IgG complexes to human mesangial cells.IgG受体和C1q受体在IgG复合物与人系膜细胞的结合增强中均起作用。
J Am Soc Nephrol. 1996 Apr;7(4):573-81. doi: 10.1681/ASN.V74573.
6
Role of complement component C1q in phagocytosis of Listeria monocytogenes by murine macrophage-like cell lines.补体成分C1q在小鼠巨噬细胞样细胞系吞噬单核细胞增生李斯特菌中的作用。
Infect Immun. 1993 Sep;61(9):3664-72. doi: 10.1128/iai.61.9.3664-3672.1993.
7
Production and characterization of a murine monoclonal IgM antibody to human C1q receptor (C1qR).一种针对人C1q受体（C1qR）的鼠单克隆IgM抗体的制备与特性分析
J Immunol. 1986 Jul 15;137(2):618-24.
8
Interaction of C1q, and other proteins containing collagen-like domains, with the C1q receptor.C1q以及其他含有胶原样结构域的蛋白质与C1q受体的相互作用。
Biochem Soc Trans. 1990 Dec;18(6):1145-8. doi: 10.1042/bst0181145.
9
Short amino acid sequences derived from C1q receptor (C1q-R) show homology with the alpha chains of fibronectin and vitronectin receptors and collagen type IV.
J Leukoc Biol. 1992 Jun;51(6):546-56. doi: 10.1002/jlb.51.6.546.
10
Detection of a high-affinity binding site for the globular head regions of the C1q complement protein on a human diploid fibroblast subtype.在一种人类二倍体成纤维细胞亚型上检测到C1q补体蛋白球状头部区域的高亲和力结合位点。
Mol Immunol. 1989 Jul;26(7):677-85. doi: 10.1016/0161-5890(89)90051-5.
引用本文的文献
1
Cell surface expression and function of the macromolecular c1 complex on the surface of human monocytes.人单核细胞表面大分子 C1 复合物的表面表达和功能。
Front Immunol. 2012 Mar 5;3:38. doi: 10.3389/fimmu.2012.00038. eCollection 2012.
2
Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli: further evidence that factor H regulates the classical complement pathway.补体蛋白 C1q 和因子 H 与脂多糖和大肠杆菌的相互作用：进一步证明因子 H 调节经典补体途径。
Protein Cell. 2011 Apr;2(4):320-32. doi: 10.1007/s13238-011-1029-y. Epub 2011 May 15.
3
Role of complement component C1q in phagocytosis of Listeria monocytogenes by murine macrophage-like cell lines.补体成分C1q在小鼠巨噬细胞样细胞系吞噬单核细胞增生李斯特菌中的作用。
Infect Immun. 1993 Sep;61(9):3664-72. doi: 10.1128/iai.61.9.3664-3672.1993.
4
Clustering of neutrophil leucocytes in serum: possible role of C1q-containing immune complexes.血清中嗜中性白细胞的聚集：含C1q免疫复合物的可能作用。
Clin Exp Immunol. 1993 Aug;93(2):237-41. doi: 10.1111/j.1365-2249.1993.tb07972.x.
5
Localization of the receptor-binding site in the collectin family of proteins.凝集素蛋白家族中受体结合位点的定位
Biochem J. 1993 Jul 1;293 ( Pt 1)(Pt 1):15-9. doi: 10.1042/bj2930015.
6
Mammalian lectins in activation and clearance mechanisms involving the complement system.参与补体系统的激活和清除机制的哺乳动物凝集素。
Springer Semin Immunopathol. 1994;15(4):307-26. doi: 10.1007/BF01837363.
7
Binding of human collectins (SP-A and MBP) to influenza virus.人凝集素（表面活性蛋白A和甘露聚糖结合蛋白）与流感病毒的结合。
Biochem J. 1994 Dec 1;304 ( Pt 2)(Pt 2):455-61. doi: 10.1042/bj3040455.
8
Identification of a gC1q-binding protein (gC1q-R) on the surface of human neutrophils. Subcellular localization and binding properties in comparison with the cC1q-R.人中性粒细胞表面gC1q结合蛋白（gC1q-R）的鉴定。与cC1q-R相比的亚细胞定位及结合特性。
J Clin Invest. 1995 Apr;95(4):1569-78. doi: 10.1172/JCI117830.
9
Antibody-independent interaction between the first component of human complement, C1, and the outer membrane of Escherichia coli D31 m4.人补体第一成分C1与大肠杆菌D31 m4外膜之间的非抗体依赖性相互作用
Biochem J. 1985 Dec 1;232(2):513-9. doi: 10.1042/bj2320513.
10
Role of platelet factors and serum complement in growth of fibroblasts with high-affinity Clq complement receptors.
In Vitro Cell Dev Biol. 1988 Jul;24(7):719-26. doi: 10.1007/BF02623611.
本文引用的文献
1
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
2
Analysis of receptor-mediated C1q binding to human peripheral blood mononuclear cells.受体介导的C1q与人类外周血单个核细胞结合的分析。
J Immunol. 1980 Oct;125(4):1658-64.
3
Evidence that C1q, a subcomponent of the first component of complement, is an Fc receptor of peritoneal and alveolar macrophages.补体第一成分的一个亚成分C1q是腹膜和肺泡巨噬细胞的Fc受体的证据。
Immunobiology. 1980 Apr;157(1):54-61. doi: 10.1016/S0171-2985(80)80062-3.
4
Immunofluorescence studies on the subcomponents of the first component of complement (C1): detection of C1q and C1s in different cells of biopsy material and on human as well as on guinea pig peritoneal macrophages.补体第一成分（C1）亚成分的免疫荧光研究：活检材料不同细胞以及人及豚鼠腹膜巨噬细胞中C1q和C1s的检测
Immunobiology. 1981;158(3):213-24. doi: 10.1016/S0171-2985(81)80071-X.
5
Characterization of the Fc receptor for IgG on a human macrophage cell line, U937.人巨噬细胞系U937上IgG的Fc受体的特性分析。
J Immunol. 1980 Dec;125(6):2735-41.
6
Interaction of C1q with DNA.C1q与DNA的相互作用。
Mol Immunol. 1982 Sep;19(9):1179-87. doi: 10.1016/0161-5890(82)90328-5.
7
Identification of types of cells in human peripheral blood that bind C1q.鉴定人类外周血中与C1q结合的细胞类型。
J Immunol. 1981 Mar;126(3):1174-9.
8
Chemical studies on the isolated collagen-like and globular fragment of complement component C1q. Comparative studies on bovine and human C1q.补体成分C1q分离出的类胶原样片段和球状片段的化学研究。牛C1q和人C1q的比较研究。
Biochim Biophys Acta. 1983 Jan 12;742(1):122-8. doi: 10.1016/0167-4838(83)90367-9.
9
MLC-conditioned media stimulate an increase in Fc receptors on human macrophages.MLC 条件培养基刺激人类巨噬细胞上 Fc 受体增加。
J Immunol. 1981 Feb;126(2):666-8.
10
Measurement of the association constants of the complexes formed between intact C1q or pepsin-treated C1q stalks and the unactivated or activated C1r2C1s2 tetramers.完整的C1q或胃蛋白酶处理的C1q茎与未活化或活化的C1r2C1s2四聚体形成的复合物的缔合常数的测定。
Mol Immunol. 1983 Jan;20(1):53-66. doi: 10.1016/0161-5890(83)90105-0.
Zotero 插件