Guyre P M, Crabtree G R, Bodwell J E, Munck A
J Immunol. 1981 Feb;126(2):666-8.
Macrophage Fc receptors (FcR) are essential for antibody-dependent cellular cytotoxicity and for optimal phagocytosis of opsonized particulate antigens. Culture in the presence of conditioned medium from mixed leukocyte cultures (MLC-CM) resulted in a dose- and time-dependent increase (up to 10-fold) in FcR-dependent binding of 125I-labeled IgG1 to promyelocytic HL-60 cells, macrophage-like U-937 cells, and normal cultured human monocytes. FcR increase in HL-60 cells was blocked by cycloheximide (100 microM) and was accompanied by a slight decrease in binding affinity. Since cell volume did not change, the increase in FcR probably represents an increase in the surface density of FcR sites. MLC-CM prepared with or without serum were equally effective in augmenting FcR sites, whereas only serum-containing MLC-CM caused morphologic change of U-937 and HL-60 cells.
巨噬细胞Fc受体（FcR）对于抗体依赖性细胞毒性以及调理素化颗粒抗原的最佳吞噬作用至关重要。在混合白细胞培养物的条件培养基（MLC-CM）存在下培养，导致125I标记的IgG1与早幼粒细胞HL-60细胞、巨噬细胞样U-937细胞和正常培养的人单核细胞的FcR依赖性结合呈剂量和时间依赖性增加（高达10倍）。HL-60细胞中FcR的增加被环己酰亚胺（100 microM）阻断，并伴有结合亲和力的轻微降低。由于细胞体积没有变化，FcR的增加可能代表FcR位点表面密度的增加。含血清或不含血清制备的MLC-CM在增加FcR位点方面同样有效，而只有含血清的MLC-CM会导致U-937和HL-60细胞的形态变化。
