Raffaelli M, Fimiani M, Castelli A, Mancianti M L, Valentino A, Andreassi L
Boll Soc Ital Biol Sper. 1984 Mar 30;60(3):467-71.
The aim of this work is to identify and purify cultured nevus cells. We used for our study monoclonal antibody 225.28S. This antibody reacts with a surface antigen which is expressed by nevus cells and melanoma cells, but it does not react with normal melanocytes. We studied 10 dermic nevi and we observed that antigenic determinant survives in cultured nevus cells. These results allowed us to employ the method of panning for purify cultured nevus cells.
这项工作的目的是识别和纯化培养的痣细胞。我们在研究中使用了单克隆抗体225.28S。这种抗体与一种表面抗原发生反应，该抗原由痣细胞和黑色素瘤细胞表达，但不与正常黑素细胞发生反应。我们研究了10个皮肤痣，并且观察到抗原决定簇在培养的痣细胞中存活。这些结果使我们能够采用淘选法来纯化培养的痣细胞。
