Yanaihara N, Yanaihara C, Mochizuki T, Iwahara K, Fujita T, Iwanaga T
Peptides. 1981;2 Suppl 2:185-91. doi: 10.1016/0196-9781(81)90029-2.
Radioimmunoassay specific for GRP was developed using C-terminal specific anti-synthetic porcine GRP sera. Measurement of the porcine gastrointestine and brain tissue extracts with GRP and with bombesin radioimmunoassays indicates that the bombesin-like immunoreactivity found in the porcine tissues is attributable to GRP or GRP-like materials. GRP-like immunoreactivity in extracts of porcine gastrointestinal tissues and pancreas was shown, by gel filtration, to comprise mainly two components: the first-eluting component corresponding to synthetic GRP and the second-eluting one to synthetic GRP(14--27) in their elution volumes. An extract of the hypothalamus also contained two similar components, while extracts of the medulla oblongata and cerebral cortex contained mainly the second-eluting immunoreactivity. Immunostaining with anti-GRP sera revealed positive nerve fibers in the myenteric nerve plexus of the porcine duodenum and in intrapancreatic ganglia, but no immunoreactivity endocrine cells in the mucosa of the porcine stomach and intestine. The results gave support to GRP as a new brain-gut peptide and suggested physiological significant of GRP as a neurotransmitter or neuromodulator.
利用C末端特异性抗合成猪GRP血清开发了针对GRP的放射免疫测定法。用GRP放射免疫测定法和蛙皮素放射免疫测定法对猪的胃肠道和脑组织提取物进行测量，结果表明，在猪组织中发现的类蛙皮素免疫反应性归因于GRP或类GRP物质。通过凝胶过滤显示，猪胃肠道组织和胰腺提取物中的类GRP免疫反应性主要由两种成分组成：第一种洗脱成分对应于合成GRP，第二种洗脱成分对应于合成GRP(14 - 27)，它们具有各自的洗脱体积。下丘脑提取物也含有两种类似成分，而延髓和大脑皮层提取物主要含有第二种洗脱的免疫反应性物质。用抗GRP血清进行免疫染色显示，猪十二指肠的肌间神经丛和胰腺内神经节中有阳性神经纤维，但在猪胃和肠黏膜的内分泌细胞中未发现免疫反应性。这些结果支持GRP作为一种新的脑肠肽，并表明GRP作为神经递质或神经调节剂具有重要的生理学意义。
