人类血吸虫病中的特异性IgE抗体及含IgE的循环免疫复合物

Specific IgE antibodies and IgE containing circulating immune complexes in human schistosomiasis.

作者信息

Feldmeier H, Redeker K, Daffalla A A, Knobloch J

出版信息

Bull Soc Pathol Exot Filiales. 1983 Nov;76(5):571-8.

Abstract

IgE antibodies (IgE-AB) have been investigated in patients infected with S. mansoni, S. haematobium, S. mansoni and S. haematobium or S. intercalatum using an enzyme immuno assay based on the RAST principle. Antigens applied were crude extracts from S. mansoni, S. haematobium and S. japonicum adult worms. IgE containing immune complexe (IgE-CIC) were determined by precipitation with PEG and subsequent identification with I125 labelled antihuman IgE. In the patients with schistosomiasis IgE-AB to the homologous antigen were demonstrated in 84% of monoinfected and in 100% of the mixed-infected individuals. Within the mixed-infected patient group IgE-AB to S. mansoni antigen were significantly higher than to S. haematobium. Cross-reactivity of IgE-antibodies was proved by use of S. haematobium and S. japonicum antigen in S. mansoni monoinfection . Specifity of the test was 94%. The amount of IgE-CIC correlated significantly with the concentration of the homologous IgE-AB, indicating that these CIC were composed of specific antibodies linked to parasite antigen.

摘要

已使用基于放射变应原吸附测定（RAST）原理的酶免疫测定法，对感染曼氏血吸虫、埃及血吸虫、曼氏血吸虫和埃及血吸虫或间插血吸虫的患者的IgE抗体（IgE-AB）进行了研究。所用抗原为曼氏血吸虫、埃及血吸虫和日本血吸虫成虫的粗提物。含IgE免疫复合物（IgE-CIC）通过用聚乙二醇沉淀并随后用I125标记的抗人IgE进行鉴定来测定。在血吸虫病患者中，84%的单一感染者和100%的混合感染者体内出现了针对同源抗原的IgE-AB。在混合感染患者组中，针对曼氏血吸虫抗原的IgE-AB显著高于针对埃及血吸虫的IgE-AB。在曼氏血吸虫单一感染中，通过使用埃及血吸虫和日本血吸虫抗原证实了IgE抗体的交叉反应性。该检测的特异性为94%。IgE-CIC的量与同源IgE-AB的浓度显著相关，表明这些CIC由与寄生虫抗原相连的特异性抗体组成。