SundarRaj N, Sundar-Raj C V, Martin J
Curr Eye Res. 1984 Apr;3(4):637-44. doi: 10.3109/02713688409003065.
Several monoclonal antibodies were developed to cell-surface antigens of human corneal fibroblasts grown in culture. Indirect immunohistochemical analysis showed that the antigens recognized by these monoclonal antibodies were also present on the skin fibroblasts. However, these antigens were not detectable on corneal epithelial cells and, therefore, were specific with respect to cell-type. When screened for complement dependent cytotoxicity by dye-exclusion test and 51Cr-release assay, two of the antibodies, designated 5F2-2 (IgM type) and 7E2-1 (IgG type) were found to be cytotoxic to human embryonic lung and skin fibroblasts but not to corneal epithelial cells grown in culture. These two antibodies were utilized to selectively eliminate corneal fibroblasts in the presence of rabbit complement to establish corneal epithelial cells in culture free of corneal fibroblasts.
针对培养的人角膜成纤维细胞的细胞表面抗原开发了几种单克隆抗体。间接免疫组织化学分析表明，这些单克隆抗体识别的抗原也存在于皮肤成纤维细胞上。然而，在角膜上皮细胞上未检测到这些抗原，因此，它们在细胞类型方面具有特异性。通过染料排除试验和51Cr释放试验筛选补体依赖性细胞毒性时，发现两种抗体，即5F2-2（IgM型）和7E2-1（IgG型）对人胚胎肺和皮肤成纤维细胞具有细胞毒性，但对培养的角膜上皮细胞无细胞毒性。利用这两种抗体在兔补体存在的情况下选择性消除角膜成纤维细胞，以建立无角膜成纤维细胞的培养角膜上皮细胞。
