Beery J T, Taylor S L, Schlunz L R, Freed R C, Bergdoll M S
Infect Immun. 1984 May;44(2):234-40. doi: 10.1128/iai.44.2.234-240.1984.
Staphylococcal enterotoxin A (SEA) was administered orally (15 micrograms) to two groups of rats. A marked immune reaction was evoked in the stomach and proximal small intestine of the first group. The second group of rats was used to study the absorptive fate and sites of action of orally administered SEA, utilizing immunoperoxidase staining. After oral dosing of the second group of rats. SEA-related immunoperoxidase staining was confined to: (i) neutrophils and macrophages, principally in the duodenum, and (ii) glomerular neutrophils and cells of the proximal convoluted tubules. Peroxidase staining of the kidney was noted within 15 min of exposure, indicating that SEA or some major postabsorption antigenic product can promptly pass through an intact gastrointestinal mucous membrane and become renally localized. Intestinal and renal detoxification and removal was indicated by an absence of detectable antigen in rats 180 min postexposure. Neuronal binding of SEA in the gastrointestinal tract was not demonstrable.
将葡萄球菌肠毒素A（SEA）以口服方式（15微克）给予两组大鼠。第一组大鼠的胃和近端小肠引发了明显的免疫反应。第二组大鼠用于利用免疫过氧化物酶染色研究口服SEA的吸收命运和作用部位。给第二组大鼠口服给药后，与SEA相关的免疫过氧化物酶染色局限于：（i）主要在十二指肠的中性粒细胞和巨噬细胞，以及（ii）肾小球中性粒细胞和近端曲管细胞。在接触后15分钟内就注意到肾脏的过氧化物酶染色，这表明SEA或某些主要的吸收后抗原产物可以迅速穿过完整的胃肠道粘膜并在肾脏中定位。暴露后180分钟的大鼠中未检测到可检测的抗原，表明肠道和肾脏进行了解毒和清除。未证实SEA在胃肠道中的神经元结合。
