酵母营养细胞和子囊孢子中膜去极化剂对海藻糖酶的激活作用。
Activation of trehalase by membrane-depolarizing agents in yeast vegetative cells and ascospores.
作者信息
Thevelein J M
出版信息
J Bacteriol. 1984 Apr;158(1):337-9. doi: 10.1128/jb.158.1.337-339.1984.
Abstract
The membrane-depolarizing agents 2,4-dinitrophenol, carbonylcyanide m-chlorophenylhydrazone, and nystatin are known to cause a rapid increase in the cyclic AMP level in fungal cells. Addition of these proton ionophores to yeast stationary-phase cells or ascospores causes an immediate 10-fold increase in trehalase activity. This observation is in agreement with a role for cyclic AMP-induced phosphorylation in the activation process of trehalase. It also provides an explanation for previous results on the induction of trehalose breakdown by 2,4-dinitrophenol in resting yeast cells.
摘要
已知膜去极化剂2,4-二硝基苯酚、羰基氰化物间氯苯腙和制霉菌素可使真菌细胞中的环磷酸腺苷（cAMP）水平迅速升高。将这些质子离子载体添加到酵母静止期细胞或子囊中，可使海藻糖酶活性立即提高10倍。这一观察结果与cAMP诱导的磷酸化在海藻糖酶激活过程中的作用一致。它也为之前关于2,4-二硝基苯酚诱导静止酵母细胞中海藻糖分解的结果提供了解释。
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Does a cyclic AMP-dependent phosphorylation initiate the transfer of trehalase from the cytosol into the vacuoles in Saccharomyces cerevisiae?环磷酸腺苷（cAMP）依赖性磷酸化是否启动了酿酒酵母中海藻糖酶从细胞质向液泡的转运？
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