Bab I, Ashton B A, Owen M E, Boyde A
J Microsc. 1984 Apr;134(Pt 1):49-53. doi: 10.1111/j.1365-2818.1984.tb00503.x.
To eliminate sectioning artefacts, we devised a method for the preparation of surfaces of plastic embedded hard tissues and their examination by incident light fluorescence microscopy. Flat surfaces produced by cutting on a microtome or using a low-speed saw were stained with dyes in aqueous solutions. Best results were obtained in tissues embedded in glycol-methacrylate. This polymer allowed good penetration of the dyes and its optical properties render cutting defects invisible with the present method. The relationship between hard and soft tissues was well preserved and the integrity of the mineralized component maintained. Cellular details and the distinction between osteoid and bone could be clearly/demonstrated. The procedure is simple, rapid and produces good results even in instances when histological sectioning is unsatisfactory.
为消除切片伪像，我们设计了一种制备塑料包埋硬组织表面的方法，并通过落射光荧光显微镜对其进行检查。用切片机切割或使用低速锯制作的平面，用染料的水溶液进行染色。在乙二醇甲基丙烯酸酯包埋的组织中获得了最佳结果。这种聚合物能使染料良好渗透，并且其光学性质使切割缺陷在本方法下不可见。硬组织和软组织之间的关系得到了很好的保留，矿化成分的完整性得以维持。细胞细节以及类骨质和骨之间的区别可以清晰地显示出来。该方法简单、快速，即使在组织学切片不理想的情况下也能产生良好的效果。
