Urinary 6-keto PGF1 alpha and PGE2 in two kidney-one clip hypertension in the rat.

The 24 hour urinary excretion of 6-keto PGF1 alpha and PGE2 was compared in 2 kidney-1 clip rats developing hypertension within 12 weeks of renal artery clipping with rats remaining normotensive over this period. Although systolic blood pressure was markedly elevated in the hypertensive (210 +/- 5.1 mm Hg), in contrast with the normotensive (141 +/- 1.9 mm Hg) group, urinary levels of 6-keto PGF1 alpha (26.1 +/- 3.4 and 22.1 +/- 2.7 ng/24 h, respectively) and PGE2 (52.8 +/- 28 and 53.3 +/- 10.8 ng/24 h) were not significantly different. Treating the 2 kidney-1 clip normotensive group with indomethacin (3.0 mg/kg, by intraperitoneal injection) twice-weekly for 3 weeks reduced 6-keto PGF1 alpha excretion from 22.1 +/- 2.7 to 8.4 +/- 2.2 ng/24 h (P less than 0.002) and PGE2 from 53.3 +/- 10.8 to 8.7 +/- 1.8 ng/24 h (P less than 0.002) but did not change blood pressure when compared with a similar group given buffer vehicle only. These findings do not support a role for renal prostaglandins in 2 kidney-1 clip hypertension in the rat.