Tavares C A, de Rossi R, Payares G, Simpson A J, McLaren D J, Smithers S R
Z Parasitenkd. 1984;70(2):189-97. doi: 10.1007/BF00942221.
A monoclonal antibody has been raised by immunizing a mouse with an isolated tegumental preparation of adult Schistosoma mansoni. The hybridoma designated NIMP/M.47, secreted an IgG2a antibody which was positive by indirect immunofluorescence with live schistosomula of S. mansoni, but not with live schistosomula of S. bovis, or with other living life cycle stages of S. mansoni. In complement-dependent, or cell-mediated in vitro cytotoxicity assays, the monoclonal antibody mediated levels of schistosomular killing as high as those obtained with sera from infected mice. No significant protection, however, was obtained in passive transfer experiments. NIMP/M47 was specific for a 20,000 dalton polypeptide in the schistosomular surface, which was also recognized by serum from infected mice.
用曼氏血吸虫成虫的分离皮层制备物免疫小鼠，产生了一种单克隆抗体。命名为NIMP/M.47的杂交瘤分泌一种IgG2a抗体，该抗体通过间接免疫荧光法检测，与曼氏血吸虫活的童虫呈阳性反应，但与牛血吸虫活的童虫或曼氏血吸虫其他生命周期阶段的活体无阳性反应。在补体依赖或细胞介导的体外细胞毒性试验中，该单克隆抗体介导的童虫杀伤水平与感染小鼠血清所达到的水平一样高。然而，在被动转移实验中未获得显著的保护作用。NIMP/M47对童虫表面一种20,000道尔顿的多肽具有特异性，感染小鼠的血清也能识别该多肽。
