Toledo V P, Tavares C A, Henriques S B, Nascimento E, Resende M
Departamento de Parasitologia, Instituto de Ciências Biológicas da UFMG, Brasil.
Rev Inst Med Trop Sao Paulo. 1994 May-Jun;36(3):199-204. doi: 10.1590/s0036-46651994000300002.
An IgG2a subclass monoclonal antibody, C6G9, was obtained by immunization of BALB/c mice with Schistosoma mansoni egg antigens. With this monoclonal antibody, it was possible to identify a schistosomular antigen with a molecular weight of 46 kilodaltons (KDa), and its expression being evaluated by means of indirect immunofluorescence. The antigen persisted in the integument of the developing schistosomulum, for at least 96 hours post-transformation. The monoclonal antibody also reacted with the cercaria surface, but not with that of adult worm. The C6G9 was also able to mediate significant levels of cytotoxicity in the presence of complement for newly transformed schistosomula.
通过用曼氏血吸虫卵抗原免疫BALB/c小鼠，获得了一种IgG2a亚类单克隆抗体C6G9。利用这种单克隆抗体，能够鉴定出一种分子量为46千道尔顿（KDa）的血吸虫抗原，并通过间接免疫荧光法评估其表达情况。该抗原在发育中的血吸虫体表持续存在，转化后至少96小时。该单克隆抗体也与尾蚴表面发生反应，但不与成虫表面反应。在补体存在的情况下，C6G9还能够介导对新转化的血吸虫显著水平的细胞毒性。
