Murty M V, Venkitasubramanian T A
Ann Microbiol (Paris). 1983 Nov-Dec;134B(3):359-65.
The growth and synthesis of macromolecules in spheroplasts of Mycobacterium smegmatis ATCC 14468 were found to be in a balanced state. Spheroplasts were not lysed during preparation and subsequent incubation, and were not contaminated with whole cells. Limited growth of spheroplasts was not due to depletion of medium constituents. High molar concentrations of osmotic stabilizer did not inhibit balanced growth. These studies establish that spheroplasts are metabolically active and can be used as a tool in cell wall synthesis and in genetic studies.
耻垢分枝杆菌ATCC 14468原生质体中大分子的生长和合成处于平衡状态。原生质体在制备和后续培养过程中未发生裂解，也未被完整细胞污染。原生质体的有限生长并非由于培养基成分的耗尽。高摩尔浓度的渗透稳定剂不会抑制平衡生长。这些研究表明，原生质体具有代谢活性，可作为细胞壁合成和基因研究的工具。
