脾毛细胞的体外培养。
In vitro culture of spleen hairy cells.
作者信息
Carli P M, Turc-Carel C, Lorenzini J L, Devilliers E, Bonhomme J, Marie J P
出版信息
Biomed Pharmacother. 1983;37(9-10):471-3.
PMID:6372886
Abstract
In vitro culture of hairy cells were successful in 5 patients with hairy cell leukemia: splenic cells were cultured in methyl cellulose plus PHA-leucocyte conditioned stimulation medium for 7 days. Plating efficiency was 0.5% with colony cells having morphological and cytochemical characteristics of hairy cells. A linear correlation between the number of cells plated and the number of colonies produced was found for cell concentrations varying from 5.10(4) to 5.10(5).
摘要
5例毛细胞白血病患者的毛细胞体外培养成功：脾细胞在甲基纤维素加PHA - 白细胞条件刺激培养基中培养7天。接种效率为0.5%，集落细胞具有毛细胞的形态学和细胞化学特征。对于5×10⁴至5×10⁵的细胞浓度，发现接种的细胞数量与产生的集落数量之间存在线性关系。
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