Gel'fand V I, Ivanova O Iu, Mittel'man L A, Pletiushkina O Iu
Biull Eksp Biol Med. 1984 Apr;97(4):491-4.
Small fragments of the peripheral cytoplasm were obtained from cytochalasine B-treated mouse embryo fibroblasts and studied for distribution of microtubules by indirect immunofluorescence. Microtubules were demonstrated to progressively depolymerize in these fragments which did not contain any tubules after 6 hours of incubation in the growth medium. This effect was specific for microtubules, since the distribution of intermediate filaments remained unchanged during incubation. The fragments remained viable during incubation, inasmuch no changes were detectable in the membrane potential of the mitochondria stained with rhodamine 123. Progressive destruction of microtubules in the tiny cell fragments is likely to be related to the lack of centrioles in such fragments.
从小鼠胚胎成纤维细胞经细胞松弛素B处理后获得外周细胞质的小片段，并通过间接免疫荧光研究微管的分布。结果表明，在生长培养基中孵育6小时后，这些片段中的微管逐渐解聚，孵育后不含任何微管。这种作用对微管具有特异性，因为中间丝的分布在孵育过程中保持不变。在孵育过程中，这些片段仍保持活力，因为用罗丹明123染色的线粒体膜电位未检测到变化。微小细胞片段中微管的逐渐破坏可能与这些片段中缺乏中心粒有关。
