van der Heide S, Kauffman H F, de Vries K
J Allergy Clin Immunol. 1984 Jun;73(6):813-8. doi: 10.1016/0091-6749(84)90452-4.
IgG and IgE antibody titers against Aspergillus fumigatus were measured in patient sera by enzyme immunoassays by use of antigens or allergens immobilized to different carriers. Specific-IgG antibodies were measured by a double antibody-layer enzyme immunoassay; specific IgE was determined by Phadezym -RAST (Pharmacia Diagnostics). In both cases antigens and allergens were immobilized in two ways: first by covalent binding to CNBr-activated paper discs and second by spontaneous binding to polystyrene surface of microtiter plates. Much higher IgE-antibody titers were found with allergens immobilized to paper discs when these discs were compared with microtiter plates, which could be explained by a higher allergen-binding capacity of activated paper discs. On the contrary, higher IgG antibody titers were found with antigens bound to microtiter plates when these plates were compared with paper discs. It is concluded that IgG antibodies are directed against antigenic components that are preferentially bound to polystyrene surfaces.
通过酶免疫测定法，使用固定于不同载体上的抗原或变应原，对患者血清中抗烟曲霉的IgG和IgE抗体滴度进行测定。特异性IgG抗体通过双抗体层酶免疫测定法进行测定；特异性IgE通过Phadezym -RAST（Pharmacia诊断公司）进行测定。在这两种情况下，抗原和变应原均以两种方式固定：第一种是通过与溴化氰活化的纸片共价结合，第二种是通过自发结合到微量滴定板的聚苯乙烯表面。当将固定于纸片上的变应原与微量滴定板进行比较时，发现前者的IgE抗体滴度要高得多，这可以通过活化纸片具有更高的变应原结合能力来解释。相反，当将与纸片进行比较时，发现与微量滴定板结合的抗原具有更高的IgG抗体滴度。得出的结论是，IgG抗体针对的是优先结合于聚苯乙烯表面的抗原成分
