Stull T L, Jacobs R F, Haas J E, Roberts M C, Wilson C B, Smith A L
J Gen Microbiol. 1984 Mar;130(3):665-72. doi: 10.1099/00221287-130-3-665.
We examined bactericidal and opsonizing activity of pooled adult 'immune' serum against Haemophilus influenzae type b with and without the addition of phagocytes. Four type b strains from cerebrospinal fluid (CSF) and three such strains from the nasopharynx (NP) of healthy children were examined. Duplicate reaction mixtures contained organisms in exponential (E) or stationary phase (S) of growth, serum, a complement source (human agammaglobulinaemic serum), and culture medium (bactericidal assay); separate assays contained the above components and polymorphonuclear leucocytes (opsonization system). A decrease in bacterial density of greater than or equal to 1 log10 unit was considered significant. All four S-CSF strains, three of four E-CSF strains and one of three S-NP strains were sensitive to the bactericidal activity of pooled serum. The other E-CSF strain, two S-NP strains and all three E-NP strains were resistant to the bactericidal activity of pooled serum. Two of three E-NP strains were opsonized by pooled serum; the other strains resistant to the bactericidal activity of pooled serum were also resistant to opsonization. Bactericidal and opsonizing activity of serum from an immunized adult was greater than or equal to that of pooled serum against each strain. Assuming normal adults are immune to invasive H. influenzae type b infection, an experimental test reflecting this immunity is the bactericidal activity against CSF isolates tested in stationary phase. We conclude that protection against invasive disease due to H. influenzae type b appears more complex than the presence of bactericidal and opsonizing activity in serum.
我们检测了成年“免疫”混合血清在添加和不添加吞噬细胞的情况下对b型流感嗜血杆菌的杀菌和调理活性。检测了来自健康儿童脑脊液（CSF）的4株b型菌株和来自鼻咽（NP）的3株此类菌株。一式两份的反应混合物包含处于生长指数期（E）或稳定期（S）的细菌、血清、补体来源（人丙种球蛋白缺乏血清）和培养基（杀菌试验）；单独的试验包含上述成分和多形核白细胞（调理系统）。细菌密度降低大于或等于1个对数10单位被认为具有显著性。所有4株稳定期脑脊液菌株、4株指数期脑脊液菌株中的3株以及3株稳定期鼻咽菌株中的1株对混合血清的杀菌活性敏感。另一株指数期脑脊液菌株、2株稳定期鼻咽菌株和所有3株指数期鼻咽菌株对混合血清的杀菌活性耐药。3株指数期鼻咽菌株中的2株被混合血清调理；其他对混合血清杀菌活性耐药的菌株对调理也耐药。免疫成年个体血清的杀菌和调理活性大于或等于混合血清对每种菌株的活性。假设正常成年人对侵袭性b型流感嗜血杆菌感染具有免疫力，反映这种免疫力的一项实验检测是针对稳定期检测的脑脊液分离株的杀菌活性。我们得出结论，针对b型流感嗜血杆菌所致侵袭性疾病的保护作用似乎比血清中存在杀菌和调理活性更为复杂。
