Tischler M E, Cammisa H
Metabolism. 1984 Jun;33(6):515-20. doi: 10.1016/0026-0495(84)90005-2.
Soft tissue injury to one hindlimb of rats was used to test the metabolic response of atrial and ventricular muscle to trauma. Effects of insulin on muscle metabolism were also studied. In myocytes and atria from normal animals, insulin increased protein synthesis and decreased protein degradation. For myocytes of rats at one and two days after trauma, this effect of insulin on proteolysis could not be detected. Over the next two days, the inhibitory effect returned to normal. Insulin also did not increase protein synthesis on day 1, but did thereafter. In atria, in contrast to heart cells, the inhibitory effect of insulin on proteolysis was enhanced at two and three days after trauma, and its stimulation of protein synthesis was unaltered. Insulin increased carbohydrate metabolism in both myocytes and atria of normal rats and traumatized rats after 2 days, and trauma did not alter this response. In myocytes, but not atria, trauma led to a faster oxidation of leucine and a significant rise in the production of alanine. Production of glutamine and glutamate was not affected in either tissue. These results show that the metabolic responses to trauma of atrial and ventricular muscle differ considerably.
利用大鼠一侧后肢的软组织损伤来测试心房和心室肌对创伤的代谢反应。还研究了胰岛素对肌肉代谢的影响。在正常动物的心肌细胞和心房中，胰岛素增加蛋白质合成并减少蛋白质降解。对于创伤后1天和2天的大鼠心肌细胞，未检测到胰岛素对蛋白水解的这种作用。在接下来的两天里，抑制作用恢复正常。胰岛素在第1天也没有增加蛋白质合成，但此后增加了。与心肌细胞相反，在心房中，创伤后2天和3天胰岛素对蛋白水解的抑制作用增强，而其对蛋白质合成的刺激作用未改变。胰岛素在创伤后2天增加了正常大鼠和创伤大鼠心肌细胞和心房中的碳水化合物代谢，创伤并未改变这种反应。在心肌细胞而非心房中，创伤导致亮氨酸氧化加快，丙氨酸生成显著增加。两种组织中谷氨酰胺和谷氨酸的生成均未受影响。这些结果表明，心房肌和心室肌对创伤的代谢反应有很大差异。
