A study on the mutagenic effect of some Bulgarian drugs using the Salmonella/microsome test.

It has been shown that mixtures of phenobarbital and 3- methylcholantrene may be used in the Salmonella/microsome test as inductors of the S9 liver fraction, instead of "Aroclor 1254". The Salmonella/microsome method has been used to study the mutagenic action of Flatogel (2.5% polymethylsyloxanum activatum and 2% ethylium -p- aminobenzoicum , suspended in sorbitol-balanced gel of aluminum hydroxidatum and magnesium hydroxidatum ), Nitrolong (2,2-bis/hydroxymethyl/-1,3- propandioltetranitrate ) and Cymethidine (N-cyano-N'-methyl-N"/2-/5-methyl-4-imidazolyl-methylthio/ethyl/guanidin e). These substances are produced as drugs by the Bulgarian chemical and pharmaceutical industry ( PHARMACHIM ). The results obtained show that the drugs studied have no mutagenic action, both without and with activation with S9 liver fraction isolated from rats treated with "Aroclor 1254" or combinations of phenobarbital and 3- methylcholantrene .