Anthony E L, Gustafson A W
Am J Anat. 1984 May;170(1):101-15. doi: 10.1002/aja.1001700108.
Pituitary gonadotropes were identified throughout the year in the seasonally breeding, hibernating bat Myotis lucifugus lucifugus by means of light microscopic immunohistochemistry. In both male and female bats, these cells were immunoreactive with an antiserum directed to the beta subunit of luteinizing hormone. Some gonadotropes were aggregated near a portion of the infundibular stalk which crosses the anterior lobe, while most were scattered singly in a uniform manner throughout the rest of the pars distalis. This cell population exhibited seasonal variations in both sexes. In males, the proportional volume of the pars distalis occupied by immunoreactive gonadotropes (volume fraction) was significantly reduced in late July, when plasma testosterone levels were approaching their seasonal peak. In females, the volume fraction declined in April, following ovulation, and remained low during pregnancy and lactation. The size and shape of gonadotropes appeared relatively constant throughout the annual reproductive cycle in male bats; the immunoreactive cells were irregular in shape, with cytoplasmic extensions insinuating between and often "cupping" other secretory cell types. In females, the gonadotropes resembled those of males throughout most of the year, except during pregnancy, when these cells became enlarged and ovoid. No evidence of involution was observed in these anterior pituitary cells in either males or females during hibernation.
通过光学显微镜免疫组织化学方法，在季节性繁殖、冬眠的蝙蝠小棕蝠（Myotis lucifugus lucifugus）中全年都能鉴定出垂体促性腺细胞。在雄性和雌性蝙蝠中，这些细胞与针对促黄体生成素β亚基的抗血清发生免疫反应。一些促性腺细胞聚集在穿过前叶的漏斗柄的一部分附近，而大多数则以均匀的方式单个散在于远侧部的其余部分。这一细胞群体在两性中均表现出季节性变化。在雄性中，当血浆睾酮水平接近季节性峰值时，即7月下旬，免疫反应性促性腺细胞所占远侧部的比例体积（体积分数）显著降低。在雌性中，排卵后的4月体积分数下降，在怀孕和哺乳期间一直保持较低水平。在雄性蝙蝠的整个年度生殖周期中，促性腺细胞的大小和形状相对恒定；免疫反应性细胞形状不规则，其细胞质延伸插入并常常“包围”其他分泌细胞类型之间。在雌性中，除了怀孕期间这些细胞变大并呈卵形外，一年中的大部分时间促性腺细胞与雄性相似。在冬眠期间，无论是雄性还是雌性，这些垂体前叶细胞均未观察到退化迹象。
