Desantis S, Labate M, Corriero A, Labate G M, De Metrio G
Department of Zoology, University of Bari, Italy.
Eur J Histochem. 2000;44(4):385-95.
The pars distalis from the pituitary gland of adult male ruin lizards (Podarcis sicula campestris De Betta), captured during the five periods of the annual sexual cycle (emergence from hibernation, reproductive period, summer regression, autumnal recrudescence, winter arrest), was studied immunohistochemically using specific antibodies against hFSHbeta, hLHbeta, oFSHbeta, and oLHbeta with the avidin-biotin complex (ABC) procedure to monitor the seasonal changes in shape, size and percentage area taken up from gonadotropes. FSH containing cells were specifically identified with anti-hFSHbeta and anti-oFSHbeta sera, whereas the LH cells were localized by anti-hLHbeta. The anti-oLHbeta serum showed cross-reactivity with the cells immunostained by the three above antisera (anti-hFSHbeta, anti-oFSHbeta, and anti-hLHbeta). None of the cells contained both gonadotropic hormones as shown by the double-immunostaining procedure. Generally, FSH cells were larger and more numerous than LH cells. FSH cells were elongated or pyriform in shape from spring to autumn, whereas they were round or oval during the winter stasis and until the emergence from hibernation. The size and the percentage area occupied by FSH cells showed an annual pattern with two distinct peaks in the reproductive and in the autumnal recrudescence periods. LH cells did not show seasonal changes in shape, being round or oval throughout the reproductive cycle, whereas their size and the area they occupied underwent seasonal variations. The LH cells reached the largest size during the reproductive period and the smallest size during the summer regression. The percentage area occupied by LH cells in the pars distalis peaked at the emergence from hibernation and during the summer refractory period, when FSH cells displayed their lowest values.
在成年雄性废墟蜥蜴（Podarcis sicula campestris De Betta）一年中的五个性周期阶段（从冬眠中苏醒、繁殖期、夏季消退期、秋季再发育期、冬季停滞期）捕获样本，取其垂体远侧部，采用抗人促卵泡激素β亚基（hFSHβ）、抗人促黄体生成素β亚基（hLHβ）、抗蜥蜴促卵泡激素β亚基（oFSHβ）和抗蜥蜴促黄体生成素β亚基（oLHβ）的特异性抗体，运用抗生物素蛋白-生物素复合物（ABC）法进行免疫组织化学研究，以监测促性腺细胞的形状、大小和所占面积百分比的季节性变化。含促卵泡激素的细胞通过抗hFSHβ和抗oFSHβ血清特异性鉴定，而促黄体生成素细胞则通过抗hLHβ定位。抗oLHβ血清与上述三种抗血清（抗hFSHβ、抗oFSHβ和抗hLHβ）免疫染色的细胞呈现交叉反应。双重免疫染色法显示，没有细胞同时含有两种促性腺激素。一般来说，促卵泡激素细胞比促黄体生成素细胞更大且数量更多。从春季到秋季，促卵泡激素细胞呈细长形或梨形，而在冬季停滞期直至从冬眠中苏醒时，它们呈圆形或椭圆形。促卵泡激素细胞的大小和所占面积百分比呈现出年度模式，在繁殖期和秋季再发育期有两个明显的峰值。促黄体生成素细胞的形状没有季节性变化，在整个生殖周期中均为圆形或椭圆形，但其大小和所占面积会发生季节性变化。促黄体生成素细胞在繁殖期达到最大尺寸，在夏季消退期达到最小尺寸。垂体远侧部中促黄体生成素细胞所占面积百分比在从冬眠中苏醒时以及夏季不应期达到峰值，此时促卵泡激素细胞的值最低。
