从微球菌中血清学分离葡萄球菌的快速检测方法。
Rapid test for the serological separation of staphylococci from micrococci.
作者信息
Seidl P H, Schleifer K H
出版信息
Appl Environ Microbiol. 1978 Mar;35(3):479-82. doi: 10.1128/aem.35.3.479-482.1978.
A simple test for the serological separation of staphylococci from micrococci is described, which is based on the quite different cell wall peptidoglycan structures of these two genera. Antisera to (pentaglycyl-epsilon-amino-n-hexanoic acid)20-albumin agglutinated without exception all staphylococci and gave no positive reaction with micrococci or other bacterial cells. To obtain a good reaction, it was necessary to extract the cells with hot trichloroacetic acid for 30 min. Antisera to (tri-L-alanyl-epsilon-amino-n-hexanoic acid)22-albumin reacted strongly with micrococci containing oligo-L-alanine bridges in their peptidoglycan, but did not agglutinate staphylococci or other bacteria lacking alanine interpeptide bridges.
本文描述了一种基于葡萄球菌属和微球菌属细胞壁肽聚糖结构差异显著的血清学方法，用于从微球菌中分离葡萄球菌。抗（五甘氨酰-ε-氨基正己酸）20-白蛋白血清无一例外地凝集所有葡萄球菌，而与微球菌或其他细菌细胞无阳性反应。为获得良好反应，有必要用热三氯乙酸提取细胞30分钟。抗（三-L-丙氨酰-ε-氨基正己酸）22-白蛋白血清与肽聚糖中含有寡聚-L-丙氨酸桥的微球菌强烈反应，但不凝集葡萄球菌或其他缺乏丙氨酸肽间桥的细菌。
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