Faller A, Schleifer K H
J Clin Microbiol. 1981 Jun;13(6):1031-5. doi: 10.1128/jcm.13.6.1031-1035.1981.
Two simple and rapid methods for the separation of staphylococci from micrococci are described. They are based on modified oxidase and benzidine tests. Micrococci and Staphylococcus sciuri yield a blue color with a 6% solution of tetramethylphenylenediamine in dimethyl sulfoxide, whereas all of the other staphylococci exhibit no coloration. Best's results were obtained with overnight cultures on blood agar. The presence of c-type cytochromes i micrococci and S. sciuri could be detected with benzidine; all noncovalently linked heme groups are removed before the addition of the benzidine reagent. The oxidase test is the simplest and most rapid method for the separation of staphylococci (except S. sciuri) from micrococci, if the nutritional requirements and the time of incubation are strictly followed. This test is especially recommended for the examination of clinical material in which S. sciuri is usually not found.
本文描述了两种从微球菌中分离葡萄球菌的简单快速方法。它们基于改良的氧化酶和联苯胺试验。微球菌和松鼠葡萄球菌在含6%四甲基对苯二胺的二甲基亚砜溶液中呈蓝色，而其他所有葡萄球菌均无颜色变化。在血琼脂上培养过夜可获得最佳结果。用联苯胺可检测微球菌和松鼠葡萄球菌中c型细胞色素的存在；在加入联苯胺试剂之前，所有非共价连接的血红素基团均被去除。如果严格遵循营养需求和培养时间，氧化酶试验是从微球菌中分离葡萄球菌（松鼠葡萄球菌除外）的最简单、最快速的方法。该试验特别推荐用于通常不含松鼠葡萄球菌的临床材料检查。
