Jourdan P S, Mansell R L, Oliver D G, Weiler E W
Anal Biochem. 1984 Apr;138(1):19-24. doi: 10.1016/0003-2697(84)90762-0.
A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpene-lactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forced-air microplate incubator.
本文描述了一种用于定量测定柑橘汁样品中苦味三萜内酯类柠檬苦素的固相酶免疫分析法。该方法可检测低至0.1 ppm的柠檬苦素。在整个分析时间1小时内即可获得定量结果。该分析方法使用了具有高免疫反应性的柠檬苦素-碱性磷酸酶示踪剂，并已通过抗体包被的聚苯乙烯微量比色皿、垂直光路光度计和强制空气微孔板培养箱实现了半自动化。
