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Plant Physiol. 1985 Apr;77(4):896-902. doi: 10.1104/pp.77.4.896.
2
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Planta Med. 1982 Feb;44(2):82-6. doi: 10.1055/s-2007-971407.
3
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[UPLC-Q-TOF-MS analysis of naringin and naringenin and its metabolites in rat plasma after intragastrical administration of alcohol extract of exocarpium Citri grandis].[柚皮苷和柚皮素及其代谢产物在大鼠灌胃给予柚皮乙醇提取物后血浆中的超高效液相色谱-四极杆飞行时间质谱分析]
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本文引用的文献

1
Radioimmunoassay for the citrus bitter principle, naringin, and related flavonoid-7-O-neohesperidosides.柑橘苦味物质柚皮苷及相关黄酮-7-O-新橙皮糖苷的放射免疫分析。
Planta Med. 1982 Feb;44(2):82-6. doi: 10.1055/s-2007-971407.
2
Assay of plasma insulin in human subjects by immunological methods.采用免疫学方法检测人体受试者血浆胰岛素。
Nature. 1959 Nov 21;184 (Suppl 21):1648-9. doi: 10.1038/1841648b0.
3
The preparation of antigenic hapten-carrier conjugates: a survey.抗原性半抗原-载体缀合物的制备：综述
Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
4
Radioimmunoassays: an overview.放射免疫分析：概述
Methods Enzymol. 1980;70(A):201-9.
5
Production of reagent antibodies.试剂抗体的生产。
Methods Enzymol. 1980;70(A):104-42. doi: 10.1016/s0076-6879(80)70044-7.
6
Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus.用于定量柑橘中柠檬苦素的竞争性固相酶联免疫分析
Anal Biochem. 1984 Apr;138(1):19-24. doi: 10.1016/0003-2697(84)90762-0.
7
Amphetamine radioimmunoassay: chemical tuning of specificity.苯丙胺放射免疫分析：特异性的化学调整
J Immunoassay. 1983;4(1):83-98. doi: 10.1080/15321818308057001.
8
A chromatographic-fluorometric method for determination of naringin, naringenin rutinoside, and related flavanone blycosides in grapefruit juice and juice sacs.一种用于测定葡萄柚汁和囊瓣中柚皮苷、柚皮素芸香糖苷及相关黄烷酮糖苷的色谱荧光法。
Anal Biochem. 1965 Sep;12(3):472-82. doi: 10.1016/0003-2697(65)90213-7.
9
Radioimmunoassay: a probe for the fine structure of biologic systems.放射免疫测定法：一种用于生物系统精细结构的探测方法。
Science. 1978 Jun 16;200(4347):1236-45. doi: 10.1126/science.208142.

柚皮苷在柑橘组织中的含量：I. 用于柚皮苷放射免疫分析的不同抗体和示踪剂的比较。

Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin.

机构信息

Department of Biology, University of South Florida, Tampa, Florida 33620.

出版信息

Plant Physiol. 1985 Apr;77(4):896-902. doi: 10.1104/pp.77.4.896.

DOI:10.1104/pp.77.4.896
PMID:16664158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1064627/
Abstract

The preparation of a tritiated radiotracer that was used in the radioimmunoassay of naringin (naringenin-7-O-alpha-rhamnosyl- (1-2)-beta-d-glucopyranoside) and which was synthesized by reduction of the carbonyl group of the flavanone is reported. The resulting assay has a detection limit of 0.5 picomole per 0.1 milliliter, is specific for the 7-neohesperidoside substitution on flavanones, and can measure naringin in crude extracts of plant tissues. This radioimmunoassay is compared with three other naringin immunoassays which use antibodies raised against two different haptens and different tracers labeled with (125)I or (3)H. The applicability of the methods to the quantification of naringin and other flavanone neohesperidosides in citrus tissue is discussed.

摘要

报道了一种通过还原黄烷酮的羰基合成的用于柚皮苷（柚皮素-7-O-α-鼠李糖基-（1-2）-β-D-吡喃葡萄糖苷）放射免疫分析的氚化放射性示踪剂的制备。该测定法的检测限为每 0.1 毫升 0.5 皮摩尔，对黄烷酮上的 7-新橙皮糖苷取代基具有特异性，并且可以测量植物组织粗提物中的柚皮苷。将该放射免疫测定法与使用针对两种不同半抗原和用 (125)I 或 (3)H 标记的不同示踪剂标记的抗体产生的另外三种柚皮苷免疫测定法进行了比较。讨论了这些方法在柑橘组织中柚皮苷和其他黄烷酮新橙皮糖苷定量中的适用性。