Torres-Medina A
Am J Vet Res. 1984 Apr;45(4):643-51.
Gnotobiotic calves (24 hours old) were monoinfected with calf rotavirus (CRV) strain NCDV, an enterotoxigenic Escherichia coli (ETEC) strain B44 (K99+), or a nonenterotoxigenic E coli (NETEC) strain 123 (K99-). Calves also were dually infected with CRV and either ETEC or NETEC. Eighteen calves equally allotted between 6 treatment groups were used in these studies: Noninfected controls--group A; CRV--group B; ETEC--group C; NETEC--group D; CRV + ETEC--group E; and CRV + NETEC--group F. Severe diarrhea and villous atrophy were observed in calves of treatment groups B, C, E, and F. Mortality was present only in treatment groups C and E as result of ETEC infection. There were no significant differences in the clinical responses or enteric lesions between treatments B and F, although a significant increase in the concentrations of NETEC was demonstrated in calves dually infected with CRV + NETEC (group F) as compared with calves monoinfected with NETEC (group D). Calves inoculated with ETEC (group C) had severe villous atrophy, neutrophilic infiltration of intestinal lumen, and moderate enterocyte necrosis. Calves dually inoculated with CRV + ETEC (group E) had the most extensive and severe lesions, similar to those in group C, plus a pronounced necrotic fibrino-hemorrhagic enteritis. Infection of enterocytes by CRV did not affect in any way the adherence of ETEC to the intestinal mucosa. Dual viral and bacterial infections of the same enterocytes were evident.
无菌新生犊牛（24小时龄）分别单感染犊牛轮状病毒（CRV）NCDV株、产肠毒素大肠杆菌（ETEC）B44株（K99+）或非产肠毒素大肠杆菌（NETEC）123株（K99-）。犊牛也同时感染CRV和ETEC或NETEC。在这些研究中使用了18头犊牛，平均分配到6个治疗组：未感染对照组——A组；CRV——B组；ETEC——C组；NETEC——D组；CRV + ETEC——E组；CRV + NETEC——F组。在治疗组B、C、E和F的犊牛中观察到严重腹泻和绒毛萎缩。由于ETEC感染，仅在治疗组C和E中有死亡情况。治疗组B和F之间的临床反应或肠道病变没有显著差异，尽管与单感染NETEC的犊牛（D组）相比，同时感染CRV + NETEC的犊牛（F组）中NETEC浓度显著增加。接种ETEC的犊牛（C组）有严重的绒毛萎缩、肠腔内嗜中性粒细胞浸润和中度肠上皮细胞坏死。同时接种CRV + ETEC的犊牛（E组）有最广泛和严重的病变，与C组相似，加上明显的坏死性纤维蛋白出血性肠炎。CRV对肠上皮细胞的感染丝毫没有影响ETEC对肠黏膜的黏附。同一肠上皮细胞的病毒和细菌双重感染很明显。
