Levison M A, Tsao T C, Trunkey D D
Arch Surg. 1984 Jul;119(7):803-8. doi: 10.1001/archsurg.1984.01390190045010.
Previous studies from this laboratory described myocardial depression in an arterially perfused rabbit interventricular septum following perfusion with acute septic plasma. Calcium is critical for maintenance of cardiac contractility on a beat-to-beat basis. We have investigated calcium flux in the septal tissue to determine whether altered calcium flux explains the impaired cardiac function during sepsis. Twenty-two rabbit septa were perfused with control and septic perfusate (cryo-precipitated plasma + RBCs) and calcium flux determined in seven experiments. Perfusate cations (Ca++, Na+, K+, and H+) were measured, tissue function and arterial pressure were monitored. Developed tension decreased 46%, acceleration of tension change fell 42%, and arterial pressure decreased 26%, all highly significant. All septa recovered after return to control perfusate. The septic perfusate Ca++ was significantly lower than control perfusate, while K+ and H+ were significantly elevated. Ion flux studies, however, could not demonstrate altered calcium flux associated with the depressed contractility.
该实验室之前的研究描述了在急性脓毒症血浆灌注后，动脉灌注的兔室间隔出现心肌抑制。钙对于逐搏维持心脏收缩力至关重要。我们研究了间隔组织中的钙通量，以确定钙通量的改变是否能解释脓毒症期间心脏功能受损的原因。用对照液和脓毒症灌注液（冷沉淀血浆+红细胞）灌注22个兔室间隔，并在7个实验中测定钙通量。测量灌注液中的阳离子（Ca++、Na+、K+和H+），监测组织功能和动脉压。舒张期张力降低46%，张力变化加速度降低42%，动脉压降低26%，均具有高度显著性。所有室间隔在恢复对照灌注液后均恢复。脓毒症灌注液中的Ca++显著低于对照灌注液，而K+和H+显著升高。然而，离子通量研究未能证明与收缩力降低相关的钙通量改变。
