Law R O
Biochim Biophys Acta. 1984 Jun 27;773(2):246-52. doi: 10.1016/0005-2736(84)90088-9.
Steady-state cellular water content and cation content and concentration have been studied in slices of rat renal cortex incubated in media in which Cl- was replaced by various monovalent anions at pH 7.35. Anions derived from low molecular weight aliphatic acids caused net uptake of cell water and K+. In the presence of larger anions cell water content fell in a manner related to anionic equivalent weight, but water content in media containing anions from weak aliphatic or alicyclic acids was slightly but consistently higher than that in media containing anions from strong acids. Cell K+ content did not decrease in these media. Aromatic anions caused enhanced cell water loss. When external pH adjusted to 6.8 or 7.8 it was found that in media containing anions from weak acids, but not from strong acids, increasing pH was associated with significantly decreased cell water content.
在pH值为7.35的培养基中，用各种单价阴离子取代Cl-，对培养的大鼠肾皮质切片中的稳态细胞含水量、阳离子含量和浓度进行了研究。源自低分子量脂肪酸的阴离子导致细胞水分和K+的净摄取。在存在较大阴离子的情况下，细胞含水量以与阴离子当量重量相关的方式下降，但含有弱脂肪酸或脂环酸阴离子的培养基中的含水量略高于但始终高于含有强酸阴离子的培养基中的含水量。在这些培养基中细胞K+含量没有降低。芳香族阴离子导致细胞水分流失增加。当外部pH值调整到6.8或7.8时，发现在含有弱酸而非强酸阴离子的培养基中，pH值升高与细胞含水量显著降低有关。
