魁北克死产仔猪和断奶后仔猪中脑心肌炎病毒的分离
Isolation of encephalomyocarditis virus among stillborn and post-weaning pigs in Quebec.
作者信息
Dea S A, Bilodeau R, Martineau G P
机构信息
Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Canada.
出版信息
Arch Virol. 1991;117(1-2):121-8. doi: 10.1007/BF01310497.
Encephalomyocarditis (EMC) virus was isolated from aborted fetuses and lungs of suckling pigs from three Quebec pig farms that experienced outbreaks of reproductive failure in sows and respiratory problems in suckling and post-weaning piglets. Multifocal interstitial pneumonia and mild non-suppurative myocarditis and meningoencephalitis were the more significant histopathological lesions observed in piglets. Vero cells were found to be more sensitive than BHK-21 cells and pig cell lines for primary isolation of EMC virus. The Quebec EMC virus isolates were highly virulent for mice and were antigenically related to reference strain of EMC virus as demonstrated by indirect immunofluorescence, seroneutralization and Western immunoblotting. Specific virus neutralization antibody titers up to 1:12,800 were detected in samples of thoracic or abdominal fluids of the aborted fetuses.
从魁北克三个猪场的流产胎儿和哺乳仔猪的肺中分离出脑心肌炎（EMC）病毒，这些猪场的母猪出现繁殖障碍，哺乳仔猪和断奶后仔猪出现呼吸道问题。在仔猪中观察到的更显著的组织病理学病变是多灶性间质性肺炎、轻度非化脓性心肌炎和脑膜脑炎。发现Vero细胞比BHK - 21细胞和猪细胞系对EMC病毒的初次分离更敏感。魁北克EMC病毒分离株对小鼠具有高度致病性，并且通过间接免疫荧光、血清中和和免疫印迹法证明与EMC病毒参考株具有抗原相关性。在流产胎儿的胸液或腹液样本中检测到高达1:12,800的特异性病毒中和抗体滴度。
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