利用抗结合蛋白抗体对海胆精子顶体反应进行的快速免疫测定。

Rapid immunoassays for the acrosome reaction of sea urchin sperm utilizing antibody to bindin.

作者信息

Vacquier V D

出版信息

Exp Cell Res. 1984 Aug;153(2):281-6. doi: 10.1016/0014-4827(84)90600-1.

Abstract

Two assays employing rhodamine-conjugated and peroxidase-conjugated antibindin are presented. The assays are used to detect the occurrence of the acrosome reaction in sea urchin sperm. The assays are rapid and can be performed on large numbers of samples. The scoring of the reacted and unreacted cells can be done accurately at a rate of 60 cells/min.

摘要

本文介绍了两种使用罗丹明偶联和过氧化物酶偶联抗结合蛋白的检测方法。这些检测方法用于检测海胆精子顶体反应的发生情况。这些检测方法快速，可对大量样本进行检测。已反应和未反应细胞的评分可以以每分钟60个细胞的速度准确完成。