Daniel T M, Gonchoroff N J, Katzmann J A, Olds G R
Infect Immun. 1984 Jul;45(1):52-5. doi: 10.1128/iai.45.1.52-55.1984.
Four mouse monoclonal antibodies have been developed which react with Mycobacterium tuberculosis antigen 5. Each of these monoclonal antibodies has been used to prepare immunoabsorbents, and antigen has been isolated from unheated M. tuberculosis H37Ra culture filtrate by affinity chromatography with these absorbents. Antigen thus obtained was found to be similar in tuberculin reactivity and in enzyme-linked immunosorbent assays to antigen 5 isolated from polyclonal immunoabsorbents, and the protein yields from the monoclonal immunoabsorbents were similar to those from polyclonal absorbents. Antigen recovered from monoclonal absorbents cross-reacted with antigens of Mycobacterium kansasii in delayed skin tests. Immunoelectrophoresis demonstrated that the major component of the antigens eluted from the monoclonal immunoabsorbents was mycobacterial arabinomannan. Antigen 5 was not identified in the eluates by immunoelectrophoresis. These observations support the hypothesis that antigen 5 and M. tuberculosis arabinomannan contain a single major shared epitope.
已开发出四种与结核分枝杆菌抗原5发生反应的小鼠单克隆抗体。这些单克隆抗体中的每一种都已用于制备免疫吸附剂，并且通过使用这些吸附剂的亲和色谱法从未加热的结核分枝杆菌H37Ra培养滤液中分离出抗原。发现由此获得的抗原在结核菌素反应性和酶联免疫吸附测定中与从多克隆免疫吸附剂中分离出的抗原5相似，并且单克隆免疫吸附剂的蛋白质产量与多克隆吸附剂的产量相似。从单克隆吸附剂中回收的抗原在迟发型皮肤试验中与堪萨斯分枝杆菌的抗原发生交叉反应。免疫电泳表明，从单克隆免疫吸附剂中洗脱的抗原的主要成分是分枝杆菌阿拉伯甘露聚糖。通过免疫电泳在洗脱液中未鉴定出抗原5。这些观察结果支持抗原5和结核分枝杆菌阿拉伯甘露聚糖含有单一主要共同表位的假说。
