Daniel T M, Olds G R
Clin Exp Immunol. 1985 May;60(2):249-58.
An IgM monoclonal antibody designated TB-C-1 which is broadly reactive with mycobacteria has been studied to characterize the antigens with which it reacts. Enzyme linked immunosorbent assay (ELISA) demonstrated reactivity not only with culture filtrates of several mycobacterial species but with several purified antigens of Mycobacterium tuberculosis, including protein antigens 5 and 6 and polysaccharides arabinogalactan and arabinomannan. Immunoblotting demonstrated reactivity with four distinct components of M. tuberculosis. Reactions with components of similar mol. wt were demonstrated for several other mycobacterial species, although fewer components bound with TB-C-1 in these other mycobacteria than in M. tuberculosis. Immunoabsorbents were prepared from TB-C-1 and used to isolate antigens with which the antibody reacted. Multiple antigens were identified in the eluates from M. tuberculosis, including protein antigens 6 and 7, arabinomannan, and arabinogalactan. Fewer components were recovered from other species of mycobacteria. Affinity of binding of immunoabsorbents was similar for all antigens bound. These results indicate that a common epitope is widely shared among antigens of M. tuberculosis and other mycobacteria and they suggest that species specificity of mycobacterial antigens may rest with individual epitopes rather than intact antigenic molecules.
一种名为TB-C-1的IgM单克隆抗体可与多种分枝杆菌发生广泛反应，现已对其反应的抗原进行了研究以进行特性鉴定。酶联免疫吸附测定（ELISA）表明，该抗体不仅与几种分枝杆菌的培养滤液反应，还与结核分枝杆菌的几种纯化抗原反应，包括蛋白抗原5和6以及多糖阿拉伯半乳聚糖和阿拉伯甘露聚糖。免疫印迹显示该抗体与结核分枝杆菌的四个不同组分发生反应。对于其他几种分枝杆菌，也显示出与相似分子量组分的反应，尽管与结核分枝杆菌相比，TB-C-1与这些其他分枝杆菌结合的组分较少。用TB-C-1制备免疫吸附剂，并用于分离与该抗体反应的抗原。在结核分枝杆菌的洗脱液中鉴定出多种抗原，包括蛋白抗原6和7、阿拉伯甘露聚糖和阿拉伯半乳聚糖。从其他分枝杆菌物种中回收的组分较少。所有结合抗原的免疫吸附剂结合亲和力相似。这些结果表明，结核分枝杆菌和其他分枝杆菌的抗原之间广泛共享一个共同表位，并且表明分枝杆菌抗原的物种特异性可能在于单个表位而非完整的抗原分子。
