Feria-Velasco A, Tapia-Arizmendi G, Malpica I
Arch Invest Med (Mex). 1978;9(1):67-84.
Morphological changes at the levels of both light and electron microscopy were studied regarding the secretory elements of the Harderian glands of neonatal rats, induced by environmental lighting conditions. The results were correlated with biochemical determinations of serotonin levels and hydroxyindole-O-methyl transferase (HIOMT) activity in the pineal gland. Neonatal male albino rats were divided into three groups: 12 were exposed to a light period for seven days; 12 to a dark period, and 12 to diurnal lighting conditions (light, 12 hours; darkness, 12 hours), as controls. In each group some animals were killed at 1.00 pm. The Harderian gland of each animal was immediately fixed and processed for light and electron microscopy while the pineal gland was homogenized in a fixative for biochemical determination of serotonin levels and HIOMT activity. On the light period the acinar lumen was observed to be dilated with increased secretions from the acini and excretory ducts while on the dark period the acinar lumen narrowed with hardly any secretions. On the dark period there were no changes in the cyclic variations of serotonin levels and HIOMT activity in the pineal gland; while on the light period, these cycles disappeared. It is concluded that secretory activity of the Harderian gland is modified by environmental lighting conditions, and that secretions produced by this gland appear to be directly related to the control mechanism of melatonin production by the pineal gland in neonatal rats.
研究了环境光照条件对新生大鼠哈德氏腺分泌细胞在光学显微镜和电子显微镜水平上的形态学变化。将这些结果与松果体中血清素水平和羟基吲哚 - O - 甲基转移酶（HIOMT）活性的生化测定结果进行了关联。新生雄性白化大鼠被分为三组：12只暴露于光照期7天；12只暴露于黑暗期；12只作为对照组，暴露于昼夜光照条件下（光照12小时，黑暗12小时）。在每组中，一些动物在下午1点被处死。每只动物的哈德氏腺立即固定并进行光学和电子显微镜处理，而松果体则在固定剂中匀浆，用于血清素水平和HIOMT活性的生化测定。在光照期，观察到腺泡腔扩张，腺泡和排泄管的分泌物增加；而在黑暗期，腺泡腔变窄，几乎没有分泌物。在黑暗期，松果体中血清素水平和HIOMT活性的周期性变化没有改变；而在光照期，这些周期消失。结论是，哈德氏腺的分泌活动受环境光照条件的影响，并且该腺体产生的分泌物似乎与新生大鼠松果体褪黑素产生的控制机制直接相关。
