Driver K, Lambert P A
Microbios. 1984;41(160):87-98.
Western Blotting of whole cell preparations of three strains of Proteus mirabilis after separation by electrophoresis on SDS-polyacrylamide gels revealed a complex pattern of antigens. Similar antigen profiles were obtained with isolated outer membranes indicating that the majority of cell surface antigens are located in the outer membrane. Major outer membrane proteins were strongly antigenic and cross-reactive. The highly immunogenic flagella were detected in whole cell preparations and visible in isolated outer membranes. Whereas the protein and flagellar antigens were cross-reactive, lipopolysaccharide (LPS) could only be detected as immunoreactive material using homologous antisera for each strain. The LPS appeared as two broad bands (high and low Mr, respectively) in immunoblots of whole cells, isolated outer membranes and purified LPS. However, isolated LPS could be resolved into multiple sharp bands when 4 M urea was included in the gel system. These discrete bands are assumed to represent differing O antigen chain lengths of the LPS as reported for other Gram-negative organisms.
对奇异变形杆菌三株菌株的全细胞制剂进行SDS-聚丙烯酰胺凝胶电泳分离后进行蛋白质印迹分析，结果显示出复杂的抗原模式。分离的外膜也得到了类似的抗原谱，表明大多数细胞表面抗原位于外膜。主要外膜蛋白具有强抗原性且有交叉反应性。在全细胞制剂中检测到高免疫原性的鞭毛，在分离的外膜中也可见。虽然蛋白质和鞭毛抗原具有交叉反应性，但脂多糖（LPS）只能使用各菌株的同源抗血清作为免疫反应性物质检测到。在全细胞、分离的外膜和纯化的LPS的免疫印迹中，LPS呈现为两条宽条带（分别为高分子量和低分子量）。然而，当凝胶系统中加入4M尿素时，分离的LPS可分解为多个清晰的条带。据报道，其他革兰氏阴性菌的这些离散条带被认为代表LPS不同的O抗原链长度。
