Jessop H L, Lambert P A
J Gen Microbiol. 1985 Sep;131(9):2343-8. doi: 10.1099/00221287-131-9-2343.
Serratia marcescens New CDC O14:H12 contains major outer membrane proteins of 43.5 kDal, 42 kDal (the porins) and 38 kDal (the OmpA protein) which can be separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Immunoblotting of whole cell or outer membrane preparations using antiserum raised against the whole cells revealed similar complex patterns of antigens. The OmpA protein was the major immunogen, although six other outer membrane proteins were also detected; the porins reacted only weakly with antibodies in this system. Immunoabsorption of antisera with whole cells showed that only the O antigenic chains of lipopolysaccharide and the H (flagella) antigens were accessible to antibody on the cell surface. Failure to detect the OmpA protein and other envelope antigens in this way suggests that their antigenic sites are not able to react with antibodies and are possibly masked by the O antigen.
粘质沙雷氏菌新CDC O14:H12含有43.5千道尔顿、42千道尔顿（孔蛋白）和38千道尔顿（OmpA蛋白）的主要外膜蛋白，这些蛋白可通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离。使用针对全细胞产生的抗血清对全细胞或外膜制剂进行免疫印迹，显示出相似的复杂抗原模式。OmpA蛋白是主要免疫原，尽管还检测到其他六种外膜蛋白；在该系统中，孔蛋白与抗体的反应较弱。用全细胞对抗血清进行免疫吸收表明，细胞表面抗体只能识别脂多糖的O抗原链和H（鞭毛）抗原。无法以这种方式检测到OmpA蛋白和其他包膜抗原，表明它们的抗原位点不能与抗体反应，可能被O抗原掩盖。
