Isahakia M, Alexander N J
Am J Reprod Immunol (1980). 1984 Apr-May;5(3):117-24. doi: 10.1111/j.1600-0897.1984.tb00300.x.
Antibodies to sperm and testicular autoantigens are a hallmark of vasectomy-induced murine autoimmunity. We generated five autoimmune monoclonal antibodies with spleen cells from nonimmunized but vasectomized BDF1 male mice as fusion partners in two experiments. Four of the antibodies, designated Vx 4, Vx 8, Vx 10, and Vx 23, recognize, as determined by fluorescence microscopy, topographically restricted sperm-specific antigenic determinants that appear in the germinal epithelium as spermatogenesis progresses. Antibodies Vx 8 and Vx 10 identified 60,000- and 97,000- dalton, mouse-specific sperm antigens restricted to the anterior acrosome and posterior acrosome, respectively. Antibody Vx 23 identified a 35,000- to 40,000- dalton antigen on the midpiece and postacrosomal regions of epididymal mouse sperm. The antigenic target of antibody Vx 4 on the midpiece region could not be identified with the Western blot technique. Both Vx 4 and Vx 23 cross-reacted with human, rabbit, and monkey sperm; immunofluorescence revealed that they were bound onto the midpiece region. By immunofluorescence, a single clone (Vx 24) distinctly reacted with the nuclei of somatic and testicular germ cells but not the nuclei of sperm. The isolation of this antinuclear autoantibody raises questions about the development of nonsperm-specific autoantibodies after vasectomy in the mouse.
抗精子抗体和睾丸自身抗原是输精管结扎诱导的小鼠自身免疫的标志。在两个实验中，我们以未免疫但已输精管结扎的BDF1雄性小鼠的脾细胞作为融合伙伴，产生了五种自身免疫性单克隆抗体。通过荧光显微镜检测，其中四种抗体，即Vx 4、Vx 8、Vx 10和Vx 23，识别随着精子发生进展出现在生精上皮中的拓扑学上受限的精子特异性抗原决定簇。抗体Vx 8和Vx 10分别鉴定出分子量为60,000道尔顿和97,000道尔顿的小鼠特异性精子抗原，分别局限于顶体前部和顶体后部。抗体Vx 23在附睾小鼠精子的中段和顶体后区域鉴定出一种分子量为35,000至40,000道尔顿的抗原。用蛋白质印迹技术无法鉴定抗体Vx 4在中段区域的抗原靶点。Vx 4和Vx 23均与人、兔和猴的精子发生交叉反应；免疫荧光显示它们结合在中段区域。通过免疫荧光，单个克隆（Vx 24）与体细胞和睾丸生殖细胞的细胞核有明显反应，但与精子细胞核无反应。这种抗核自身抗体的分离引发了关于小鼠输精管结扎后非精子特异性自身抗体发展的问题。
