一种由输精管结扎小鼠产生的单克隆抗精子自身抗体所识别的精子肽抗原的鉴定与表征。

Identification and characterization of a sperm peptide antigen recognized by a monoclonal antisperm autoantibody derived from a vasectomized mouse.

作者信息

Nakamura S, Tsuji Y, Nakata Y, Komori S, Koyama K

机构信息

Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.

出版信息

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1503-9. doi: 10.1006/bbrc.1994.2837.

DOI:10.1006/bbrc.1994.2837

PMID:7811229

Abstract

A monoclonal autoantibody (MAb) to mouse sperm was established from a vasectomized mouse. The MAb, termed 4A11, reacted exclusively to mouse and rat live sperm tail, without any cross-reaction with sperm of other mammalian species or any somatic organ tissues in the mouse. MAb 4A11 significantly inhibited in vitro fertilization of mouse ova. Western blotting showed a single band of 67 kDa as the corresponding antigen protein of MAb 4A11. By screening a mouse testis cDNA library with MAb 4A11, one positive clone (964bp) was obtained. DNA sequence of the clone showed no homology with any previously reported mammalian genes, but showed 54% homology with a bacterial gene of sucrose-specific phosphotransferase enzyme II. The rabbit antisera raised to the recombinant protein of the 4A11 cDNA gene showed reaction to mouse, rat, hamster and guinea-pig sperm.

摘要

从一只输精管结扎的小鼠体内制备出了一种针对小鼠精子的单克隆自身抗体（MAb）。这种单克隆抗体被命名为4A11，它仅与小鼠和大鼠的活精子尾部发生反应，与其他哺乳动物物种的精子或小鼠的任何体细胞器官组织均无交叉反应。单克隆抗体4A11显著抑制了小鼠卵子的体外受精。蛋白质免疫印迹法显示，一条67 kDa的单带是单克隆抗体4A11的相应抗原蛋白。通过用单克隆抗体4A11筛选小鼠睾丸cDNA文库，获得了一个阳性克隆（964bp）。该克隆的DNA序列与任何先前报道的哺乳动物基因均无同源性，但与一种蔗糖特异性磷酸转移酶II的细菌基因显示出54%的同源性。用4A11 cDNA基因的重组蛋白制备的兔抗血清与小鼠、大鼠、仓鼠和豚鼠的精子发生反应。