Phillips M, Hensley P, Balter R A, Cohan S L
Alcohol Clin Exp Res. 1984 May-Jun;8(3):293-6. doi: 10.1111/j.1530-0277.1984.tb05514.x.
An improved method is described for the collection of breath for the subsequent assay of acetaldehyde and other volatile components. Breath is collected in a Pyrex gas-collecting tube sealed at both ends with Teflon taps. Prior to collection or assay of the samples, this tube is heated to 72 degrees C; breath is sampled for assay by piercing a rubber septum on a sideport with the needle of a similarly heated gas-tight syringe, and injected into a gas chromatograph (GC). The advantages of this system are: (1) Avoidance of the artefacts encountered in the assay of acetaldehyde in the blood; (2) suitability for sample collection at a site remote from the GC laboratory; (3) avoidance of sample loss by leakage, contamination, or partitioning into water condensed from breath; and (4) compatibility with a "nondedicated" GC lacking any special gas-collecting circuitry. A typical study of a normal human volunteer is described, demonstrating the rise and fall of the concentration of acetaldehyde and ethanol in the breath following the ingestion of an oral dose of ethanol.
本文描述了一种改进的收集呼气的方法，用于后续乙醛及其他挥发性成分的检测。呼气收集于两端用聚四氟乙烯旋塞密封的派热克斯玻璃气体收集管中。在收集或检测样品之前，将该管加热至72摄氏度；通过用同样加热的气密注射器的针头刺穿侧口上的橡胶隔垫来采集呼气用于检测，然后注入气相色谱仪（GC）。该系统的优点包括：（1）避免了血液中乙醛检测时遇到的假象；（2）适用于在远离气相色谱实验室的地点采集样品；（3）避免因泄漏、污染或分配到呼出气冷凝水中而导致的样品损失；以及（4）与缺乏任何特殊气体收集电路的“非专用”气相色谱仪兼容。描述了一项对正常人类志愿者的典型研究，展示了口服一剂乙醇后呼气中乙醛和乙醇浓度的升降情况。
