Giglio M J, Santoro R C, Bozzini C E
Alcohol Clin Exp Res. 1984 May-Jun;8(3):323-5. doi: 10.1111/j.1530-0277.1984.tb05520.x.
Male adult mice were allowed to drink only a solution of 32% ethyl alcohol for 3 months. Hematocrit and hemoglobin concentration were lower in ethanol-treated than in control mice at the end of the experimental period. Red cell volume was not significantly different between both groups. Plasma volume was higher in experimental than in control mice. Therefore, the anemia found in ethanol-treated mice can be regarded as a dilution anemia. When ethanol-treated and control mice, both made polycythemic by hypertransfusion to suppress their endogenous erythropoietin formation, were injected with doses of erythropoietin in the range of 0.2 to 3.2 IRP units, the derived dose-response curves were markedly different because of a reduced response to the hormone by the treated mice. This finding suggests that the number of "erythropoietin-responsive cells" may be reduced as the result of ethanol, or that their response to the hormone may be delayed or inhibited. Plasma erythropoietin concentration in alcohol- treated mice, as determined in the posthypoxic polycythemic mouse bioassay, was higher than normal in both normoxic and hypoxic conditions, probably as the result of the impaired responsiveness to the hormone mentioned above.
成年雄性小鼠仅饮用32%的乙醇溶液，持续3个月。在实验期结束时，经乙醇处理的小鼠的血细胞比容和血红蛋白浓度低于对照小鼠。两组之间的红细胞体积无显著差异。实验小鼠的血浆体积高于对照小鼠。因此，在经乙醇处理的小鼠中发现的贫血可被视为稀释性贫血。当经乙醇处理的小鼠和对照小鼠通过大量输血使血细胞增多以抑制其内源性促红细胞生成素的形成后，给它们注射0.2至3.2国际参考制剂（IRP）单位范围内的促红细胞生成素剂量时，由于经处理的小鼠对该激素的反应降低，得到的剂量反应曲线明显不同。这一发现表明，“促红细胞生成素反应性细胞”的数量可能因乙醇而减少，或者它们对该激素的反应可能延迟或受到抑制。在低氧后血细胞增多小鼠生物测定中测定的经乙醇处理的小鼠的血浆促红细胞生成素浓度，在常氧和低氧条件下均高于正常水平，这可能是由于上述对该激素的反应受损所致。
