从富含蛋白酶的植物组织中提取用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的蛋白质。
Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues.
作者信息
Wu F S, Wang M Y
出版信息
Anal Biochem. 1984 May 15;139(1):100-3. doi: 10.1016/0003-2697(84)90394-4.
PMID:6377964
Abstract
A method of extracting proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from plant tissues with high protease activity was described. It resolved protein bands in high-molecular-weight regions of the gel and replaced commonly used procedures which showed severe degradation of proteins, even in the presence of protease inhibitors.
摘要
描述了一种从具有高蛋白酶活性的植物组织中提取蛋白质用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的方法。该方法能在凝胶的高分子量区域分辨出蛋白条带，取代了即使在存在蛋白酶抑制剂的情况下仍会导致蛋白质严重降解的常用方法。
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