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用于二维电泳的植物蛋白提取

Extraction of plant proteins for two-dimensional electrophoresis.

作者信息

Granier F

机构信息

Laboratoire de Génétique des Systèmes Végétaux, La Ferme du Moulon, Gif-sur-Yvette, France.

出版信息

Electrophoresis. 1988 Nov;9(11):712-8. doi: 10.1002/elps.1150091106.

DOI:10.1002/elps.1150091106
PMID:3074923
Abstract

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.

摘要

比较了用于植物蛋白质二维电泳的三种不同提取方法

(i) 用非变性Tris缓冲液提取可溶性蛋白质；(ii) 在十二烷基硫酸钠存在下于高温进行变性提取，除了回收可溶性蛋白质外，还能使膜蛋白溶解；(iii) 三氯乙酸 - 丙酮法，可直接沉淀总蛋白质。

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