Egorov N S, Krivova A Iu, Al'-Nuri M A
Antibiotiki. 1984 May;29(5):332-6.
Streptomyces spheroides 35, a novobiocin-producing organism, was used in the study. In addition to the antibiotic the culture produces proteases capable of splitting fibrin. Depending on the medium composition and component ratio different amounts of novobiocin and proteases were formed. To show various aspects of the biological interaction of the synthesis of proteolytic enzymes and novobiocin, the studies were performed with chloramphenicol. It was found that the main quantity of free amino acids accumulated in the culture liquid phase, while in the mycelial phase only traces of the amino acids were detected. The regularities of the free amino acid accumulation in the media with novobiocin and chloramphenicol were identical as compared to the control. The excess of novobiocin during the second phase of the culture development was shown to influence the synthesis of nucleic acids, whereas inhibition of protein synthesis was secondary.
球形链霉菌35，一种产生新生霉素的微生物，被用于该研究。除了抗生素外，该培养物还产生能够分解纤维蛋白的蛋白酶。根据培养基组成和成分比例，会形成不同量的新生霉素和蛋白酶。为了展示蛋白水解酶和新生霉素合成的生物相互作用的各个方面，使用氯霉素进行了研究。发现游离氨基酸的主要量积累在培养液相中，而在菌丝体相中仅检测到痕量的氨基酸。与对照相比，添加新生霉素和氯霉素的培养基中游离氨基酸积累的规律是相同的。结果表明，在培养发展的第二阶段过量的新生霉素会影响核酸的合成，而蛋白质合成的抑制是次要的。
