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DNA methylation of T3 virus ocr+ and ocr- strains in Escherichia coli cells harbouring the EcoK DNA host specificity system.

作者信息

Schroeder C, Reuter M, Krüger D H

出版信息

Biomed Biochim Acta. 1984;43(3):K1-5.

PMID:6378191
Abstract

The influence of the T3 gene functions ocr+ and sam+ on the extent of phage DNA methylation in Escherichia coli K12 cells was studied by determining the proportion of 6- methylaminopurin to adenine in the purified DNA of T3 wild-type, sam- and ocr- sam- phage strains. We demonstrate that the DNA of T3 ocr- sam- mutants carries 12 methyl groups as a result of the action of the host-specificity methylase EcoK . In contrast to this the DNA of ocr+ strains is not EcoK -specifically methylated.

摘要

相似文献

1
DNA methylation of T3 virus ocr+ and ocr- strains in Escherichia coli cells harbouring the EcoK DNA host specificity system.
Biomed Biochim Acta. 1984;43(3):K1-5.
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引用本文的文献

1
Avoidance of DNA methylation. A virus-encoded methylase inhibitor and evidence for counterselection of methylase recognition sites in viral genomes.避免DNA甲基化。一种病毒编码的甲基化酶抑制剂及病毒基因组中甲基化酶识别位点反选择的证据。
Cell Biophys. 1989 Aug-Oct;15(1-2):87-95. doi: 10.1007/BF02991582.