Forster S J, Talbot I C, Critchley D R
Br J Cancer. 1984 Jul;50(1):51-61. doi: 10.1038/bjc.1984.139.
Using an immunoperoxidase procedure, we have examined the distribution of laminin and fibronectin in normal human large intestinal mucosa and in 50 cases of rectal adenocarcinoma for which extensive clinical follow up was available. In normal tissue, laminin staining was largely restricted to basement membranes, including that underlying the epithelial cells, whereas fibronectin was found in both basement membranes and surrounding connective tissue. In rectal carcinomas, basement membrane-like staining for laminin associated with tumour cells was found in only 27 out of the 50 cases studied. Statistical analysis showed that the presence of laminin-containing basement membranes was correlated with low histological grade (well-differentiated tumours), but not with stage (progression through the bowel wall and the development of lymph node metastases) and, in a highly significant way, with a reduced incidence of distant metastases and increased patient survival. Although fibronectin was found in tumour cell basement membranes where these were present, it was also found in the stroma of all 50 tumours. There was no apparent correlation between the presence of stromal fibronectin and grade, stage or development of metastases. Finally, attention is drawn to some of the technical difficulties in detecting basement membrane antigens in formalin-fixed tissue, the material most frequently available for retrospective study.
我们采用免疫过氧化物酶法，研究了层粘连蛋白和纤连蛋白在正常人大肠黏膜以及50例有广泛临床随访资料的直肠腺癌中的分布情况。在正常组织中，层粘连蛋白染色主要局限于基底膜，包括上皮细胞下方的基底膜，而纤连蛋白则在基底膜和周围结缔组织中均有发现。在直肠癌中，在所研究的50例病例中，仅有27例发现与肿瘤细胞相关的层粘连蛋白呈基底膜样染色。统计分析表明，含层粘连蛋白的基底膜的存在与低组织学分级（高分化肿瘤）相关，但与分期（肿瘤穿透肠壁及发生淋巴结转移）无关，且与远处转移发生率降低及患者生存率提高高度相关。尽管在存在肿瘤细胞基底膜的地方发现了纤连蛋白，但在所有50例肿瘤的基质中也都发现了纤连蛋白。基质纤连蛋白的存在与分级、分期或转移的发生之间没有明显关联。最后，需要注意在福尔马林固定组织（这是回顾性研究最常用的材料）中检测基底膜抗原存在一些技术难题。
