Kannan S, Balaram P, Chandran G J, Pillai M R, Mathew B, Nalinakumari K R, Nair M K
Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India.
Histopathology. 1994 Jun;24(6):531-7. doi: 10.1111/j.1365-2559.1994.tb00571.x.
Expression of three basement membrane proteins--collagen IV, laminin and fibronectin--was studied in normal, hyperplastic, dysplastic and neoplastic conditions of the oral mucosa using immunohistochemistry. Collagen IV and laminin exhibited similar staining patterns, while fibronectin showed a different pattern of expression. The expression of collagen IV and laminin also demonstrated an inverse correlation between staining intensity, thickness and basement membrane continuity in various stages of tumour progression. In contrast to the continuous and intense staining of basement membrane in normal oral mucosa with collagen IV and laminin antibodies, severe dysplasia and carcinoma exhibited discontinuous, thin and weakly stained basement membrane. The expression of fibronectin showed a direct correlation with extent of tumour progression. In normal mucosa, expression of fibronectin was almost absent, whereas in carcinoma intense expression of fibronectin was evident in the basement membrane and basal cells. These results emphasize the value of basement membrane proteins as biological markers for assessing oral carcinogenesis.
利用免疫组织化学方法，研究了三种基底膜蛋白——IV型胶原、层粘连蛋白和纤连蛋白——在口腔黏膜正常、增生、发育异常及肿瘤状态下的表达情况。IV型胶原和层粘连蛋白呈现相似的染色模式，而纤连蛋白则表现出不同的表达模式。IV型胶原和层粘连蛋白的表达在肿瘤进展的各个阶段，在染色强度、厚度和基底膜连续性之间也呈现出负相关。与正常口腔黏膜中IV型胶原和层粘连蛋白抗体对基底膜的连续且强烈染色不同，重度发育异常和癌表现为基底膜不连续、变薄且染色较弱。纤连蛋白的表达与肿瘤进展程度呈正相关。在正常黏膜中，几乎不存在纤连蛋白的表达，而在癌组织中，基底膜和基底细胞中纤连蛋白的强烈表达很明显。这些结果强调了基底膜蛋白作为评估口腔癌发生生物学标志物的价值。
