Odom O W, Stöffler G, Hardesty B
FEBS Lett. 1984 Jul 23;173(1):155-8. doi: 10.1016/0014-5793(84)81037-6.
Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol of ribosomal protein S21. The conformational change causes an apparent decrease in distance between the probes from 59 to 52 A as determined by non-radiative energy transfer.
荧光技术被用于研究在12 mM Mg2+中加热激活时，无活性的大肠杆菌30 S核糖体亚基中发生的构象变化。激活与16 S RNA 3'-末端的一个荧光团移动到极性较小的环境中并朝向核糖体蛋白S21的半胱氨酸硫醇上的一个探针有关。这种构象变化导致通过非辐射能量转移测定的探针之间的距离从59 Å明显减小到52 Å。
